Ph-ncas9-ppe-v2
WebbpH-nCas9-PPE-V2 pSpG-PPE pH-ePPE ePPE pRN120 Sign Up for Our Newsletter. Receive the latest news, hot plasmids, discounts and more. Sign Up. Subscribe to Our Blog. Learn … WebbPlasmid pH-A3A-PBE from Dr. Caixia Gao's lab contains the insert APOBEC3A-nCas9-NLS-UGI-NLS and is published in Nat Biotechnol. 2024 Oct 1. pii: nbt.4261. doi: 10.1038/nbt.4261. This plasmid is available through Addgene.
Ph-ncas9-ppe-v2
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Webb24 mars 2024 · pH-ePPE: a plant nCas9-RT and pegRNA expression vector that includes an OsU3 promoter-driven SpCas9-based sgRNA scaffold and a ZmUbi promoter-driven … Webb24 mars 2024 · Engineered RTs, fusing functional elements or peptides to nCas9-RT (Cas9 nickase-reverse transcriptase), increasing the binding affinity of the nuclear localization signal sequences of nCas9...
Webb24 juli 2024 · Product Name : pH-nCas9-PPE-V2 article : bacterial resistance : Kanamycin cloning : backbone pHUE411 (modified from pCambia) backbone_mutation … http://www.biofeng.com/adjy/zhili/pH-nCas9-PBE.html
WebbFor sgRNA expression in monocotyledons protoplasts. Loading... 170131. pH-nCas9-PPE-V2. For plant prime editing in rice plants or monocotyledons protoplasts. Loading... http://enpcce.genetics.cas.cn/PN/CXG/SP/
WebbAbout Caixia Gao. Prof. CaixiaGao Principal Investigator, Ph.D. Research interests: Developing novel technologies to achieve efficient and specific genome engineering, and …
Webb14 mars 2024 · After incubation at room temperature for 12 h, protoplasts were harvested and homogenized in 100 μl lysis buffer containing 25 mM Tris–HCl, pH 7.8, 2 mM DTT, 2 mM trans -1,2-diaminocyclohexane- N ’ N ’ N ’ N ’-tetraacetic acid, 10% (v/v) glycerol, 1% (v/v) Triton X-100. greek marathon originWebbpH-nCas9-PPE-V2. Plasmid #170131. Purpose. For plant prime editing in rice plants or monocotyledons protoplasts. Depositor. Caixia Gao. ... Modified version of lentiSAM v2, a lenti sgRNA cloning backbone with MS2 loops at tetraloop/stemloop 2, dCas9-VP64, and puro resistance marker. flower arranging classes marylandWebbpH-nCas9-PPE-V2 Map uploaded by the depositor. Sign Up for Our Newsletter. Receive the latest news, hot plasmids, discounts and more. Sign Up. Subscribe to Our Blog. Learn about the latest plasmid technologies and research tools. Subscribe. Contact Addgene. Have questions about ... flower arranging classes minnesotaWebb16 mars 2024 · The pH-nCas9-PPE3 binary vectors were transformed into Agrobacterium tumefaciens strain EHA105 by electroporation. Callus cells of Zhonghua11 were … greek marinated baby octopusWebbpOsU3 pTaU3 pH-nCas9-PPE-V2/pH-ePPE Forward primer (5'-3'): + Spacer sequence + Reverse primer (5'-3'): + PBS and RT template sequence + Submit >> clear. Help … greek map of the worldWebbTotal vector size (bp) 6388 Vector type Plant Expression, CRISPR Growth in Bacteria Bacterial Resistance (s) Spectinomycin, 50 μg/mL Growth Temperature 37°C Growth Strain (s) DH5alpha Copy number Unknown Gene/Insert Gene/Insert name BsaI:AATG:Cas9_2:GCTT:BsaI Species Synthetic Insert Size (bp) 4141 Tag / Fusion Protein greek marinade for lamb meatWebbpH-nCas9-PPE-V2 Sequences (1) Addgene Sequences: Full (1) Full Sequences from Addgene (1) Based on next-generation sequencing (NGS) results where indicated … greek marinade for chicken breast