WebDec 25, 2024 · The requirement of TMEM67 for the gating function of the TZ that delivers membrane proteins into the ciliary compartment has not been determined. In this study, we established hTERT-RPE1 cells with knockout (KO) of TMEM67 and examined whether cilium formation and TZ gating are affected by its ablation.

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WebMounting evidence suggests that gating mechanisms regulate the selective ciliary entry of membrane and soluble proteins, whose coordinated function makes the cilium a … WebFeb 12, 2024 · Here, we show that the ciliopathy protein Rpgrip1l governs ciliary gating by ensuring the proper amount of Cep290 at the vertebrate TZ. Further, we identified the … greenwood movie where to watch

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WebAug 8, 2024 · This suggests that in sperm cilia, twitchy plays a different role to its presumed function in ciliary gating in compartmentalised cilia. In mammalian cells, five molecular components of the transition fibres have been identified from proteomic studies ( Tanos et al., 2013 ) but they have mainly been studied in primary cilia and the smaller ... WebIn particular, investigating ciliopathies has helped us to understand the molecular mechanisms by which the cilium-associated basal body functions in early ciliogenesis, as well as how the transition zone functions in ciliary gating, and how intraflagellar transport enables cargo trafficking and signalling. WebApr 1, 2012 · As such, the inappropriate ciliary accumulation of proteins may imply an active removal pathway that is defective when the TZ is disrupted. Such a bidirectional gating is reminiscent of the nuclear pore complex, whose trafficking determinants, the Ran–Importin pathway, have been implicated in some aspects of ciliary trafficking [36]. greenwood mo weather